|
|
|
David H. Slaymaker, Ph.D.
David Slaymaker (Associate
Professor) received his Ph.D in Genetics from the University of California -
Riverside in 1999 and his B.S. in Horticulture Science from Kansas State
University in 1993. Following receipt of his Ph.D, Dr. Slaymaker was a USDA
Postdoctoral Research Fellow at the Waksman Institute, Rutgers
University, NJ before joining William Paterson University
in Fall 2001. He teaches undergraduate and graduate courses in the areas of
general biology, biotechnology, and plant science.
Dr.
Slaymaker's research interests are 1) the molecular and biochemical
mechanisms of plant defense and 2) plant signal transduction and gene
regulation.
|
|
|
Schedule
|
Fall 2008 Teaching Schedule
|
|
|
MONDAY
|
TUESDAY
|
WEDNESDAY
|
THURSDAY
|
FRIDAY
|
|
9:30-10:45
|
|
|
Botany Lab
(rm S401)
|
Genetics
Lab (rm S401)
|
|
|
11:00-12:15
|
|
|
|
12:30-1:45
|
Botany Lecture
(rm S523)
|
Common Hour
|
Botany Lecture
(rm S523)
|
Common Hour
|
|
2:00-3:15
|
Office Hours
(2:00-3:30)
|
|
Office Hours
(2:00-3:30)
|
Genetics Lab
(rm S401)
|
|
|
|
3:30-4:45
|
|
|
|
|
5:00-6:15
|
|
|
|
|
|
6:30-7:45
|
|
Biotech:Proteins
(rm S514/S403)
|
|
Biotech:Proteins
(rm S514/S403)
|
|
8:00-9:15
|
|
|
Courses Taught
·
Field Biology (BIO130)
·
General Biology I (BIO163)
·
General Genetics (BIO206)
·
General Botany (BIO261)
·
Undergraduate Independent Reading (BIO497)
·
Undergraduate Independent Study (BIO499)
·
Biotech: Cell Culture (BIO531)
·
Biotech: Proteins (BIO632)
·
Graduate Independent Reading (BIO701/702)
·
Graduate Independnt Study (BIO799)
·
Detection of Genetically Modified Foods Using
PCR (BIO599)
|
|
|
|
Research Project
|
|
|
|
The
bacterial pathogen Pseudomonas
syringae pv. glycinea (Psg)
causes bacterial blight on soybean.
When Psg carries an
active copy of avirulence gene D (AvrD),
the bacterial cells produce a low molecular weight acyl-glycoside known
as the syringolide elicitor. The
syringolide elicitor specifically induces a defense response on soybean
cultivars that carry resistance gene Rpg4. Research in our lab aims to determine
the role of three proteins in the AvrD:Rpg4-dependent defense response:
GmEFH, GmGRP, and P34. GmEFH is a
plant-specific calcium-binding protein, GmGRP is a glycine-rich
RNA-binding protein, and P34 is the syringolide elicitor-binding protein. GmEFH and GmGRP were identified as
proteins that co-migrated with 2D-gel spots representing proteins that
were phosphorylated specifically during the syringolide-induced defense
response (Slaymaker and Keen, 2004).
P34 was identified and characterized by Ji et al. (1997 and 1998)
as the syringolide-binding protein and putative elicitor-receptor. Our immediate goal is to investigate a
functional role for these three proteins using RNAi-mediated gene
silencing and protein overproduction in transgenic soybean plants.
|
|
|
|
Current Research
Students
·
Kaitlin Tilney (U)
|
Past Research
Students
·
Luis Posadas
(U), Nicole Fantauzzi (U), Phu Dinh (U), Chris DeNude (U),Christian
Montes (U), Vincent DePaola (U), Suzan DelaCruz (U), Katie Banaszewsky
(U), Troy Parra (U), Issam Khairullah (U), Samira Ziaei (G), Craig Hoppey
(U)
|
|
|
Publications
|
|
Slaymaker,
D.H. and Hoppey, C.M. 2006. Reduced polysome levels and preferential
recruitment of a defense gene transcript into polysomes in soybean cells
treated with the syringolide elicitor. Plant Science 170(1): 54-60.
|
|
|
Slaymaker,
D.H. and Keen, N.T. 2004. Syringolide elicitor-induced oxidative burst
and protein phosphorylation in soybean cells, and tentative
identification of two affected phosphoproteins. Plant Science 166(2):
387-396.
|
|
|
Slaymaker,
D.H., Navarre, D.A., Clark, D., del Pozo, O., Martin, G.B. and Klessig,
D.F. 2002. The tobacco salicylic acid-binding protein 3 (SABP) is the
chloroplast carbonic anhydrase, which exhibits antioxidant activity and
plays a role in the hypersensitive defense response. Proc. Natl. Acad.
Sci. USA 99(18):11640-11645.
|
|
|
Klessig,
D.F., Kachroo, P., Slaymaker, D., Yoshioka, K., Navarre,
D.A., Kumar, D., and Shah, J. 2002. SA- and NO-mediated signaling in
plant disease resistance. In: Biology of Plant-Microbe Interactions, Vol
3. Leong, S.A.,
Allen, C., and Triplet E.W. eds. ISMPMI Press, St. Paul, Minn.
pp. 78-82.
|
|
|
Slaymaker,
D., and Keen, N.T. 2000. Perception of the syringolide elicitors by
soybean cells. In: Delivery and perception of pathogen signals in plants.
N. Keen, S. Mayama, J. Leach, and S. Tsuyumu eds. APS Press, St. Paul, Minn.
|
|
|
Ji, C.,
Boyd, C., Slaymaker, D., Okinaka, Y., Takeuchi, Y., Midland, S.L., Sims,
J.J., Herman, E., and Keen, N.T. 1998. Characterization of a 34-kDa
soybean binding protein for the syringolide elicitors. Proc. Natl. Acad.
Sci USA 95(6):3306-3311.
|
|
|
Ji, C.,
Okinaka, Y., Takeuchi, Y., Tsurushima, T., Buzzel, R.I., Sims, J.J.,
Midland, S.L., Slaymaker, D., Yoshikawa, M., Yamaoka, N., and Keen, N.T.
1997. Specific binding of the syringolide elicitors to a soluble protein
fraction from soybean leaves. Plant Cell 9(8): 1425-1433.
|
|
|
Yucel,
I., Slaymaker, D., Boyd, C., Murillo, J., Buzzel, R.I.,
and Keen, N.T. 1994. Avirulence gene avrPphC from Pseudomonas
syringae pv. phaseolicola 3121 - a plasmid-borne homologue of avrC
closely linked to an avrD allele. MPMI 7(5):677-679.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
